high content laser based spinning disk confocal microscope (Revvity)
96
Structured Review
Revvity
high content laser based spinning disk confocal microscope
High Content Laser Based Spinning Disk Confocal Microscope, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 2516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+content+laser+based+spinning+disk+confocal+microscope/Opera+Phenix+Plus+high-content+screening+system/pmc13022406-282-26-35
Average 96 stars, based on 2516 article reviews
High Content Laser Based Spinning Disk Confocal Microscope, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 2516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+content+laser+based+spinning+disk+confocal+microscope/Opera+Phenix+Plus+high-content+screening+system/pmc13022406-282-26-35
Average 96 stars, based on 2516 article reviews
high content laser based spinning disk confocal microscope - by Bioz Stars,
2026-09
96/100 stars
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Microscopy:Article Title: Protocol to profile spatially resolved NLRP3 inflammasome complexes using APEX2-based proximity labeling. Article Snippet: Liang et al., STAR Protocols 5, 103417 December 20, 2024 a 2024 The NLRP3 inflammasome is a key multi-protein complex controlling inflammation, particularly interleukin-1b (IL-1b) production.. Here, we present a protocol to profile spatially resolved NLRP3 inflammasome complexes using ascorbic peroxidase 2 (APEX2)-based proximity labeling combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS).. We describe steps for design and generation of the fusion construct, characterization of the stable FLAGNLRP3-APEX2 expression cell line by western blotting/imaging, biotinylated proteome enrichment, and mass spectrometry analysis. Article Title: A cytoplasmic motif in HLA-E that drives clathrin-mediated endocytosis and VCP-associated postendocytic trafficking Article Snippet: Briefly, proximity labeling was performed as described above before fixation with 4% paraformaldehyde, followed by permeabilization using 0.2% Triton-X (Sigma, T9284) in DPBS for 10 min at 25 °C. .. After blocking with 1% BSA in DPBS for 1 to 2 h at 25 °C, cells were incubated with anti-flag antibody (Sigma, F1804) and IRDye 800CW Streptavidin antibody (LI-COR, 926-32230) overnight at 4 °C, followed by incubation with AF647-conjugated goat anti-mouse secondary antibody (Invitrogen, A32728) at RT for 1 h. The plate was imaged on a Article Title: Protocol to profile spatially resolved NLRP3 inflammasome complexes using APEX2-based proximity labeling Article Snippet: .. Image the plate on a Article Title: Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus. Article Snippet: .. Cells were subsequently incubated with FBS-free medium containing 0.015 μM FrMLV-RBD-AF555 at room temperature for 1 hour, washed three times with PBS, and imaged on a Article Title: Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus Article Snippet: .. Cells were subsequently incubated with FBS-free medium containing 0.015 μM FrMLV-RBD-AF555 at room temperature for 1 h, washed three times with PBS, and imaged on a Article Title: Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus Article Snippet: .. Cells were subsequently incubated with FBS-free medium containing 0.015 μM FrMLV-RBD-AF555 at room temperature for 1 hour, washed three times with PBS, and imaged on a Blocking Assay:Article Title: A cytoplasmic motif in HLA-E that drives clathrin-mediated endocytosis and VCP-associated postendocytic trafficking Article Snippet: Briefly, proximity labeling was performed as described above before fixation with 4% paraformaldehyde, followed by permeabilization using 0.2% Triton-X (Sigma, T9284) in DPBS for 10 min at 25 °C. .. After blocking with 1% BSA in DPBS for 1 to 2 h at 25 °C, cells were incubated with anti-flag antibody (Sigma, F1804) and IRDye 800CW Streptavidin antibody (LI-COR, 926-32230) overnight at 4 °C, followed by incubation with AF647-conjugated goat anti-mouse secondary antibody (Invitrogen, A32728) at RT for 1 h. The plate was imaged on a Incubation:Article Title: A cytoplasmic motif in HLA-E that drives clathrin-mediated endocytosis and VCP-associated postendocytic trafficking Article Snippet: Briefly, proximity labeling was performed as described above before fixation with 4% paraformaldehyde, followed by permeabilization using 0.2% Triton-X (Sigma, T9284) in DPBS for 10 min at 25 °C. .. After blocking with 1% BSA in DPBS for 1 to 2 h at 25 °C, cells were incubated with anti-flag antibody (Sigma, F1804) and IRDye 800CW Streptavidin antibody (LI-COR, 926-32230) overnight at 4 °C, followed by incubation with AF647-conjugated goat anti-mouse secondary antibody (Invitrogen, A32728) at RT for 1 h. The plate was imaged on a Article Title: Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus. Article Snippet: .. Cells were subsequently incubated with FBS-free medium containing 0.015 μM FrMLV-RBD-AF555 at room temperature for 1 hour, washed three times with PBS, and imaged on a Article Title: Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus Article Snippet: .. Cells were subsequently incubated with FBS-free medium containing 0.015 μM FrMLV-RBD-AF555 at room temperature for 1 h, washed three times with PBS, and imaged on a Article Title: Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus Article Snippet: .. Cells were subsequently incubated with FBS-free medium containing 0.015 μM FrMLV-RBD-AF555 at room temperature for 1 hour, washed three times with PBS, and imaged on a |