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high content laser based spinning disk confocal microscope  (Revvity)


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    Structured Review

    Revvity high content laser based spinning disk confocal microscope
    High Content Laser Based Spinning Disk Confocal Microscope, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 2516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+content+laser+based+spinning+disk+confocal+microscope/Opera+Phenix+Plus+high-content+screening+system/pmc13022406-282-26-35
    Average 96 stars, based on 2516 article reviews
    high content laser based spinning disk confocal microscope - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Microscopy:

    Article Title: Protocol to profile spatially resolved NLRP3 inflammasome complexes using APEX2-based proximity labeling.
    Article Snippet: Liang et al., STAR Protocols 5, 103417 December 20, 2024 a 2024 The NLRP3 inflammasome is a key multi-protein complex controlling inflammation, particularly interleukin-1b (IL-1b) production.. Here, we present a protocol to profile spatially resolved NLRP3 inflammasome complexes using ascorbic peroxidase 2 (APEX2)-based proximity labeling combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS).. We describe steps for design and generation of the fusion construct, characterization of the stable FLAGNLRP3-APEX2 expression cell line by western blotting/imaging, biotinylated proteome enrichment, and mass spectrometry analysis.

    Article Title: A cytoplasmic motif in HLA-E that drives clathrin-mediated endocytosis and VCP-associated postendocytic trafficking
    Article Snippet: Briefly, proximity labeling was performed as described above before fixation with 4% paraformaldehyde, followed by permeabilization using 0.2% Triton-X (Sigma, T9284) in DPBS for 10 min at 25 °C. .. After blocking with 1% BSA in DPBS for 1 to 2 h at 25 °C, cells were incubated with anti-flag antibody (Sigma, F1804) and IRDye 800CW Streptavidin antibody (LI-COR, 926-32230) overnight at 4 °C, followed by incubation with AF647-conjugated goat anti-mouse secondary antibody (Invitrogen, A32728) at RT for 1 h. The plate was imaged on a high-content laser-based spinning disk confocal microscope (Opera Phenix Plus, Revvity), using a 60× water objective. .. Image analysis, including colocalization quantification, was performed in Fiji/ImageJ using the Coloc2 plug-in.

    Article Title: Protocol to profile spatially resolved NLRP3 inflammasome complexes using APEX2-based proximity labeling
    Article Snippet: .. Image the plate on a high-content laser-based spinning disk confocal microscope (Opera Phenix Plus, Revvity), using a 60× water objective. ..

    Article Title: Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus.
    Article Snippet: .. Cells were subsequently incubated with FBS-free medium containing 0.015 μM FrMLV-RBD-AF555 at room temperature for 1 hour, washed three times with PBS, and imaged on a high-content laser-based spinning disk confocal microscope (Opera Phenix Plus, Revvity) using a 60x water objective. ..

    Article Title: Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus
    Article Snippet: .. Cells were subsequently incubated with FBS-free medium containing 0.015 μM FrMLV-RBD-AF555 at room temperature for 1 h, washed three times with PBS, and imaged on a high-content laser-based spinning disk confocal microscope (Opera Phenix Plus, Revvity) using a 60x water objective. ..

    Article Title: Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus
    Article Snippet: .. Cells were subsequently incubated with FBS-free medium containing 0.015 μM FrMLV-RBD-AF555 at room temperature for 1 hour, washed three times with PBS, and imaged on a high-content laser-based spinning disk confocal microscope (Opera Phenix Plus, Revvity) using a 60x water objective. ..

    Blocking Assay:

    Article Title: A cytoplasmic motif in HLA-E that drives clathrin-mediated endocytosis and VCP-associated postendocytic trafficking
    Article Snippet: Briefly, proximity labeling was performed as described above before fixation with 4% paraformaldehyde, followed by permeabilization using 0.2% Triton-X (Sigma, T9284) in DPBS for 10 min at 25 °C. .. After blocking with 1% BSA in DPBS for 1 to 2 h at 25 °C, cells were incubated with anti-flag antibody (Sigma, F1804) and IRDye 800CW Streptavidin antibody (LI-COR, 926-32230) overnight at 4 °C, followed by incubation with AF647-conjugated goat anti-mouse secondary antibody (Invitrogen, A32728) at RT for 1 h. The plate was imaged on a high-content laser-based spinning disk confocal microscope (Opera Phenix Plus, Revvity), using a 60× water objective. .. Image analysis, including colocalization quantification, was performed in Fiji/ImageJ using the Coloc2 plug-in.

    Incubation:

    Article Title: A cytoplasmic motif in HLA-E that drives clathrin-mediated endocytosis and VCP-associated postendocytic trafficking
    Article Snippet: Briefly, proximity labeling was performed as described above before fixation with 4% paraformaldehyde, followed by permeabilization using 0.2% Triton-X (Sigma, T9284) in DPBS for 10 min at 25 °C. .. After blocking with 1% BSA in DPBS for 1 to 2 h at 25 °C, cells were incubated with anti-flag antibody (Sigma, F1804) and IRDye 800CW Streptavidin antibody (LI-COR, 926-32230) overnight at 4 °C, followed by incubation with AF647-conjugated goat anti-mouse secondary antibody (Invitrogen, A32728) at RT for 1 h. The plate was imaged on a high-content laser-based spinning disk confocal microscope (Opera Phenix Plus, Revvity), using a 60× water objective. .. Image analysis, including colocalization quantification, was performed in Fiji/ImageJ using the Coloc2 plug-in.

    Article Title: Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus.
    Article Snippet: .. Cells were subsequently incubated with FBS-free medium containing 0.015 μM FrMLV-RBD-AF555 at room temperature for 1 hour, washed three times with PBS, and imaged on a high-content laser-based spinning disk confocal microscope (Opera Phenix Plus, Revvity) using a 60x water objective. ..

    Article Title: Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus
    Article Snippet: .. Cells were subsequently incubated with FBS-free medium containing 0.015 μM FrMLV-RBD-AF555 at room temperature for 1 h, washed three times with PBS, and imaged on a high-content laser-based spinning disk confocal microscope (Opera Phenix Plus, Revvity) using a 60x water objective. ..

    Article Title: Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus
    Article Snippet: .. Cells were subsequently incubated with FBS-free medium containing 0.015 μM FrMLV-RBD-AF555 at room temperature for 1 hour, washed three times with PBS, and imaged on a high-content laser-based spinning disk confocal microscope (Opera Phenix Plus, Revvity) using a 60x water objective. ..



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